Composite

Part:BBa_K545003

Designed by: Grenoble 2011   Group: iGEM11_Grenoble   (2011-09-20)


LacI repressor followed by pLac promoter regulating MerR and CinR

this part is composed by a RBS-LacI that will repress the pLac promoter assembly in downstream of this part. The pLac promoter control two genes, MerR a regulator of transcription (K346001) and CinR a quorum sensing receptor (R0034 C0077)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1165
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2386
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None